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RPS20

ANTIBODY INFORMATION
 
 

Antibody HPA003570

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003570  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

 3  

Proper citation

 Atlas Antibodies Cat#HPA003570, RRID:AB_1856466  

VALIDATION SUMMARY
 
 

Antibody HPA003570

  
Immunocytochemistry

Approved  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003570

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum.  

Antibody dilution

 1:8  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003570

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:50  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003570

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 16, 13.4  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA003570

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA003570

  

Antigen

 Recombinant protein fragment  

Length (aa)

 117  

Antigen sequence

 
MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKV
KGPVRMPTKTLRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQ
ITSISIEPGVEVEVTIA
 

Matching transcripts

 RPS20-002 - ENSP00000009589 [100%]
RPS20-003 - ENSP00000427788 [100%]
RPS20-001 - ENSP00000429374 [95%]
RPS20-201 - ENSP00000478703 [95%]
 

Other gene match

  
ANTIGEN VIEW
RPS20-001
RPS20-002
RPS20-003
RPS20-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA003570

  

1.

 Posttranscriptional down-regulation of small ribosomal subunit proteins correlates with reduction of 18S rRNA in RPS19 deficiency
Badhai J et al
FEBS Lett 2009;583(12):2049-53
  

2.

 Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C et al
J Proteomics 2012;75(7):2236-51
  

3.

 Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18
Zhang X et al
Nat Struct Mol Biol 2015;22(5):404-10
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.