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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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CD4
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    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

CD4

Gene description

CD4 molecule

Protein class

CD markers
FDA approved drug targets
Predicted intracellular proteins
Predicted membrane proteins
Predicted secreted proteins

Predicted localization

Intracellular,Membrane,Secreted

Number of transcripts

3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Group enriched (HEL, HL-60, NB-4, THP-1, U-937)
HPA (normal tissue):Tissue enhanced (parathyroid gland)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Selective cytoplasmic expression in peripheral lymphocytes and subsets of cells in lymphoid tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. External characterization data supports antibody staining.

Reliability score

Supported based on 4 antibodies.
HPA004252 , HPA004472 , CAB000011 , CAB068180
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PROGNOSTIC SUMMARY

Prognostic marker in endometrial cancer (favourable) and renal cancer (unfavourable).
Endometrial cancer p<0.001
Renal cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Lymphoma

STAINING SUMMARY

HPA004252
HPA004472
CAB000011
CAB068180

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A majority of the malignant cells were negative. Some malignant lymphomas, gliomas and colorectal cancers displayed weak cytoplasmic immunoreactivity.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Malignant cells were generally negative. Malignant gliomas and renal cancer as well as a few cases of malignant lymphomas, ovarian, endometrial and pancreatic cancer showed weak to moderate cytoplasmic positivity.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

All cancer cells were negative. Strong cytoplasmic staining was seen in subsets of leukocytes.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues were negative.

GENE INFORMATION

Gene name

CD4

Synonyms

Description

CD4 molecule (HGNC Symbol)

Entrez gene summary

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Chromosome

12

Cytoband

p13.31

Chromosome location (bp)

6786858 - 6820808

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000010610 (version 83.38)

Entrez gene

920

UniProt

P01730 (UniProt - Evidence at protein level)

neXtProt

NX_P01730

Antibodypedia

CD4 antibodies

PROTEIN BROWSER

CD4-001
CD4-005
CD4-007

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

CD4-001
ENSP00000011653
ENST00000011653
P01730 [Direct mapping]
T-cell surface glycoprotein CD4
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CD markers
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
FDA approved drug targets
   Biotech drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001618 [virus receptor activity]
GO:0001816 [cytokine production]
GO:0001948 [glycoprotein binding]
GO:0002250 [adaptive immune response]
GO:0004872 [receptor activity]
GO:0004888 [transmembrane signaling receptor activity]
GO:0005201 [extracellular matrix structural constituent]
GO:0005515 [protein binding]
GO:0005769 [early endosome]
GO:0005788 [endoplasmic reticulum lumen]
GO:0005789 [endoplasmic reticulum membrane]
GO:0005886 [plasma membrane]
GO:0006948 [induction by virus of host cell-cell fusion]
GO:0006955 [immune response]
GO:0007155 [cell adhesion]
GO:0007165 [signal transduction]
GO:0007166 [cell surface receptor signaling pathway]
GO:0007167 [enzyme linked receptor protein signaling pathway]
GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway]
GO:0008270 [zinc ion binding]
GO:0009897 [external side of plasma membrane]
GO:0009986 [cell surface]
GO:0010524 [positive regulation of calcium ion transport into cytosol]
GO:0015026 [coreceptor activity]
GO:0016021 [integral component of membrane]
GO:0016032 [viral process]
GO:0019865 [immunoglobulin binding]
GO:0019899 [enzyme binding]
GO:0019901 [protein kinase binding]
GO:0030217 [T cell differentiation]
GO:0030260 [entry into host cell]
GO:0031295 [T cell costimulation]
GO:0032355 [response to estradiol]
GO:0032507 [maintenance of protein location in cell]
GO:0033280 [response to vitamin D]
GO:0035397 [helper T cell enhancement of adaptive immune response]
GO:0042101 [T cell receptor complex]
GO:0042102 [positive regulation of T cell proliferation]
GO:0042110 [T cell activation]
GO:0042289 [MHC class II protein binding]
GO:0042803 [protein homodimerization activity]
GO:0045058 [T cell selection]
GO:0045086 [positive regulation of interleukin-2 biosynthetic process]
GO:0045087 [innate immune response]
GO:0045121 [membrane raft]
GO:0045234 [protein palmitoleylation]
GO:0045860 [positive regulation of protein kinase activity]
GO:0046718 [viral entry into host cell]
GO:0050690 [regulation of defense response to virus by virus]
GO:0050731 [positive regulation of peptidyl-tyrosine phosphorylation]
GO:0050829 [defense response to Gram-negative bacterium]
GO:0050850 [positive regulation of calcium-mediated signaling]
GO:0050852 [T cell receptor signaling pathway]
GO:0050863 [regulation of T cell activation]
GO:0050870 [positive regulation of T cell activation]
GO:1990782 [protein tyrosine kinase binding]
Show all
458 aa
51.1 kDa
Yes 1
CD4-005
ENSP00000445167
ENST00000541982
F5H480 [Direct mapping]
T-cell surface glycoprotein CD4
Show all
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
GO:0007155 [cell adhesion]
GO:0016021 [integral component of membrane]
Show all
148 aa
16.5 kDa
No 0
CD4-007
ENSP00000440720
ENST00000539492
F5GYA9 [Direct mapping]
T-cell surface glycoprotein CD4
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
45 aa
4.8 kDa
Yes 0

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