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AKAP8L

ANTIBODY INFORMATION
 
 

Antibody HPA042485

  

Antibody HPA042546

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042485   HPA042546  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA042485, RRID:AB_2678019   Atlas Antibodies Cat#HPA042546, RRID:AB_2678046  

VALIDATION SUMMARY
 
 

Antibody HPA042485

  

Antibody HPA042546

  
Immunocytochemistry

Supported    
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042485

  

Antibody HPA042546

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles.    

Antibody dilution

 1:53    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042485

  

Antibody HPA042546

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA042546 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA042485 across 46 cell lines.		  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA042546 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA042485 across 74 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.   Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:900  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042485

  

Antibody HPA042546

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 71.6, 65.2   71.6, 65.2  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA042485

  

Antibody HPA042546

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA042485

  

Antibody HPA042546

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 99   82  

Antigen sequence

 
DEEGKEDGREEGKEDPEKGALTTQDENGQTKRKLQAGKKSQDKQKKRQRD
RMVERIQFVCSLCKYRTFYEDEMASHLDSKFHKEHFKYVGTKLPKQTAD
  
FLQEYVTNKTKKTEELRKTVEDLDGLIQQIYRDQDLTQEIAMEHFVKKVE
AAHCAACDLFIPMQFGIIQKHLKTMDHNRNRR
 

Matching transcripts

 AKAP8L-001 - ENSP00000470952 [100%]
AKAP8L-004 - ENSP00000380557 [100%]
  AKAP8L-001 - ENSP00000470952 [100%]
AKAP8L-004 - ENSP00000380557 [100%]
 

Other gene match

    
ANTIGEN VIEW
AKAP8L-001
AKAP8L-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.