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Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows distinct cytoplasmic positivity in bone marrow poietic cells.
Expression
RNA: detected in 37 tissues Protein: detected in 27 cell types
RNA: detected in 37 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:300
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041899
Antibody CAB009493
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403671)
Target mass (kDa)
12.2, 12.1, 11
15.6, 12.2, 12.1, 11
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA041899
Antibody CAB009493
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
ANTIGEN INFORMATION
Antibody HPA041899
Antibody CAB009493
Antigen
Recombinant protein fragment
Recombinant protein
Length (aa)
51
Antigen sequence
FLGRLVPRGRGAAEAATRKQRITETESPYQELQGQRSDVYSDLNTQRPYY
K