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ANTIBODY INFORMATION
 
 

Antibody HPA071285

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA071285  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 16  

References

  

Proper citation

 Atlas Antibodies Cat#HPA071285, RRID:AB_2686379  

VALIDATION SUMMARY
 
 

Antibody HPA071285

  
Immunocytochemistry

Supported  
Immunohistochemistry

Supported  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA071285

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.  

Antibody dilution

 1:4  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA071285

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human spleen shows strong nuclear membranous positivity in cells in white pulp.  

Expression

 RNA: detected in 27 tissues
Protein: detected in 19 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA071285

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 78, 71.6, 16.2  

Antibody dilution

 1:80  

PROTEIN ARRAY
 
 

Antibody HPA071285

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA071285

  

Antigen

 Recombinant protein fragment  

Length (aa)

 82  

Antigen sequence

 
MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGA
VDSYDVTVDEELGEIQLVRIEKRKYWLNDDWY
 

Matching transcripts

 ALOX5-001 - ENSP00000363512 [100%]
ALOX5-201 - ENSP00000437634 [100%]
ALOX5-202 - ENSP00000483803 [100%]
 

Other gene match

  
ANTIGEN VIEW
ALOX5-001
ALOX5-201
ALOX5-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.