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Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Antibody dilution
1:10
1:159
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA021220
Antibody HPA050722
Antibody HPA053648
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 53 cell types
RNA: detected in 37 tissues Protein: detected in 39 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:450
1:35
Literature conformity
Partly consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021220
Antibody HPA050722
Antibody HPA053648
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
73.3, 71.9, 22.3
73.3, 71.9, 29.5, 17.9
73.3, 71.9
Antibody dilution
1:250
1:100
1:800
PROTEIN ARRAY
Antibody HPA021220
Antibody HPA050722
Antibody HPA053648
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
