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Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Antibody dilution
1:50
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA030558
Antibody HPA030559
Antibody HPA030560
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA030560 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA030559 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA030560 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA030559 across 74 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human urinary bladder shows cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows cytoplasmic positivity in urothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 30 cell types
RNA: detected in 37 tissues Protein: detected in 42 cell types
RNA: detected in 37 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:30
1:115
1:30
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030558
Antibody HPA030559
Antibody HPA030560
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
89.6
89.6
89.6
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA030558
Antibody HPA030559
Antibody HPA030560
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.