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NPC1L1

ANTIBODY INFORMATION
 
 

Antibody CAB070127

  

Antibody CAB070128

  

Provider

 Novus Biologicals
   Novus Biologicals
  

Product name

 NB400-127   NB400-128  

Host species

 Rabbit   Rabbit  

Clonality

 msAb   msAb  

Purity

 Affinity   Affinity  

Other gene match

    

Released in version

 14   14  

References

    

Proper citation

 Novus Cat#NB400-127, RRID:AB_10002744   Novus Cat#NB400-128, RRID:AB_10000815  

VALIDATION SUMMARY
 
 

Antibody CAB070127

  

Antibody CAB070128

  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody CAB070127

  

Antibody CAB070128

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human small intestine shows strong luminal membranous and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows moderate cytoplasmic and membranous positivity in glandular cells.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 19 cell types		   RNA: detected in 5 tissues
Protein: detected in 28 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:800   1:900  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody CAB070127

  

Antibody CAB070128

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 148.7, 145.8, 140.4, 79.1   148.7, 145.8, 140.4, 79.1  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody CAB070127

  

Antibody CAB070128

  

Antigen

 Synthetic peptide   Synthetic peptide  

Length (aa)

    

Antigen sequence

 

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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