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Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Antibody dilution
1:43
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA035919
Antibody HPA050147
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 32 tissues Protein: detected in 46 cell types
RNA: detected in 32 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:50
Literature conformity
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035919
Antibody HPA050147
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415368)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415368)
Target mass (kDa)
34.3, 29.8
34.3
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA035919
Antibody HPA050147
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.