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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Antibody dilution
1:109
1:137
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA031231
Antibody HPA031232
Antibody HPA031233
Antibody HPA031234
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 48 cell types
RNA: detected in 37 tissues Protein: detected in 59 cell types
RNA: detected in 37 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:150
1:25
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031231
Antibody HPA031232
Antibody HPA031233
Antibody HPA031234
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418772)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
46.3, 43.6
46.3, 43.6, 34.9
46.3, 43.6, 34.9, 17.2
46.3, 43.6, 20.2
Antibody dilution
1:250
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA031231
Antibody HPA031232
Antibody HPA031233
Antibody HPA031234
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
