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Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
Antibody dilution
1:100
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA008157
Antibody HPA064664
Standard validation
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows distinct nuclear membranous positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008157
Antibody HPA064664
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
346.9, 285.8, 66.2
346.9, 285.8
Antibody dilution
1:250
1:330
PROTEIN ARRAY
Antibody HPA008157
Antibody HPA064664
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
