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Immunohistochemical staining of human hippocampus shows distinct positivity in neuropil.
Immunohistochemical staining of human hippocampus shows positivity of neuropil.
Expression
RNA: detected in 36 tissues Protein: detected in 27 cell types
RNA: detected in 36 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004823
Antibody CAB004592
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
240.9, 220.9, 210.9, 201.1, 171.4, 67
240.9, 220.9, 210.9, 201.1, 171.4, 67, 9.9, 2.4
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA004823
Antibody CAB004592
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.