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RRP12

ANTIBODY INFORMATION
 
 

Antibody HPA049945

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049945  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 12  

References

  

Proper citation

 Atlas Antibodies Cat#HPA049945, RRID:AB_2680965  

VALIDATION SUMMARY
 
 

Antibody HPA049945

  
Immunocytochemistry

Supported  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049945

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli.  

Antibody dilution

 1:85  

Literature conformity

 The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA049945

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 143.7, 137.1, 132.7  

Antibody dilution

 1:420  

PROTEIN ARRAY
 
 

Antibody HPA049945

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3400  

ANTIGEN INFORMATION
 
 

Antibody HPA049945

  

Antigen

 Recombinant protein fragment  

Length (aa)

 100  

Antigen sequence

 
NTLKSKAMDLAQAGSTVESKIYDTLQWQMWTLLPGFCTRPTDVAISFKGL
ARTLGMAISERPDLRVTVCQALRTLITKGCQAEADRAEVSRFAKNFLPIL
 

Matching transcripts

 RRP12-007 - ENSP00000446184 [100%]
RRP12-008 - ENSP00000324315 [100%]
RRP12-201 - ENSP00000360031 [100%]
RRP12-202 - ENSP00000414863 [100%]
 

Other gene match

  
ANTIGEN VIEW
RRP12-007
RRP12-008
RRP12-201
RRP12-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.