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THRAP3

ANTIBODY INFORMATION
 
 

Antibody HPA012041

  

Antibody HPA063765

  

Antibody CAB017472

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA012041   HPA063765   sc-48779  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 4   14   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA012041, RRID:AB_1857983   Atlas Antibodies Cat#HPA063765, RRID:AB_2685117   Santa Cruz Biotechnology Cat#sc-48779, RRID:AB_2271759  

VALIDATION SUMMARY
 
 

Antibody HPA012041

  

Antibody HPA063765

  

Antibody CAB017472

  
Immunocytochemistry


Validated

Validated
  

Validated
   Supported  
Immunohistochemistry

Supported     Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA012041

  

Antibody HPA063765

  

Antibody CAB017472

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		      

     

Figure description

 Nuclear region of segmented cells in 10x-images      

Antibody dilution

 1:141      
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA063765.		  
Validated

Antibody staining overlaps with antibody HPA012041.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.   Immunofluorescent staining of human cell line SiHa shows localization to nuclear speckles.   Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.  

Antibody dilution

 1:143   1:167   1:75  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012041

  

Antibody HPA063765

  

Antibody CAB017472

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.     Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		     RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:250     1:300  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012041

  

Antibody HPA063765

  

Antibody CAB017472

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

      

Target mass (kDa)

 108.7   108.7   108.7, 4.5  

Antibody dilution

 1:250   1:550   1:500  

PROTEIN ARRAY
 
 

Antibody HPA012041

  

Antibody HPA063765

  

Antibody CAB017472

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:6650    

ANTIGEN INFORMATION
 
 

Antibody HPA012041

  

Antibody HPA063765

  

Antibody CAB017472

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 131   83    

Antigen sequence

 
ETEEREESTTGFDKSRLGTKDFVGPSERGGGRARGTFQFRARGRGWGRGN
YSGNNNNNSNNDFQKRNREEEWDPEYTPKSKKYYLHDDREGEGSDKWVSR
GRGRGAFPRGRGRFMFRKSSTSPKWAHDKFS
  
RGKRSEGGHRGFVPEKNFRVTAYKAVQEKSSSPPPRKTSESRDKLGAKGD
FPTGKSSFSITREAQVNVRMDSFDEDLARPSGL
  

											 
																				

																		

										
											
Matching transcripts

												
																					THRAP3-001 - ENSP00000346634 [100%] 
THRAP3-002 - ENSP00000433825 [100%] 

											 
																						THRAP3-001 - ENSP00000346634 [100%] 
THRAP3-002 - ENSP00000433825 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
THRAP3-001
THRAP3-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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