Expression of SPAG4 in cancer - Summary - The Human Protein Atlas

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SPAG4

TISSUE

CELL

PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary


Level of antibody staining/expression
	High Medium Low Not detected

GENERAL INFORMATION
Gene name	SPAG4
Gene description	Sperm associated antigen 4
Protein class	Predicted intracellular proteins Predicted membrane proteins
Predicted localization	Intracellular,Membrane
Number of transcripts	3
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Cell line enhanced (EFO-21, U-87 MG)
HPA (normal tissue):	Mixed

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in a few tissues, including spermatids in testis.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly not consistent with RNA expression data. Pending external verification.
Reliability score	Approved based on 2 antibodies. HPA048393 , HPA061789
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PROGNOSTIC SUMMARY
Prognostic marker in endometrial cancer (favourable), colorectal cancer (unfavourable), glioma (unfavourable), renal cancer (unfavourable) and urothelial cancer (favourable).
Endometrial cancer p<0.001 Colorectal cancer p<0.001 Glioma p<0.001 Renal cancer p<0.001 Urothelial cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer

Prostate cancer

STAINING SUMMARY

HPA048393	HPA061789

GENE INFORMATION
Gene name	SPAG4 (HGNC Symbol)
Synonyms	CT127, SUN4
Description	Sperm associated antigen 4 (HGNC Symbol)
Entrez gene summary	The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development. [provided by RefSeq, Jul 2008]
Chromosome	20
Cytoband	q11.22
Chromosome location (bp)	35615892 - 35621049
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000061656 (version 83.38)
Entrez gene	6676
UniProt	Q9NPE6 (UniProt - Evidence at protein level)
neXtProt	NX_Q9NPE6
Antibodypedia	SPAG4 antibodies

PROTEIN BROWSER

SPAG4-001	SPAG4-002	SPAG4-007

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
SPAG4-001 ENSP00000363391 ENST00000374273	Q9NPE6 [Direct mapping] Sperm-associated antigen 4 protein Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 3TM proteins predicted by MDM Protein evidence (Kim et al 2014) Show all	GO:0005198 [structural molecule activity] GO:0005515 [protein binding] GO:0005635 [nuclear envelope] GO:0005737 [cytoplasm] GO:0005856 [cytoskeleton] GO:0006998 [nuclear envelope organization] GO:0007283 [spermatogenesis] GO:0016021 [integral component of membrane] GO:0031514 [motile cilium] GO:0043495 [protein anchor] GO:0090286 [cytoskeletal anchoring at nuclear membrane] Show all	437 aa 48.2 kDa	No	3
SPAG4-002 ENSP00000396670 ENST00000454819	C9JJZ6 [Direct mapping] Sperm-associated antigen 4 protein Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Show all	GO:0016021 [integral component of membrane] Show all	215 aa 24.6 kDa	Yes	1
SPAG4-007 ENSP00000399231 ENST00000430878	Q5JX49 [Direct mapping] Sperm-associated antigen 4 protein Show all	Predicted intracellular proteins Show all		141 aa 15.6 kDa	No	0

Contact

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The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.