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PMS1

ANTIBODY INFORMATION
 
 

Antibody HPA031013

  

Antibody CAB010233

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA031013   sc-615  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 16   3  

References

    

Proper citation

 Atlas Antibodies Cat#HPA031013, RRID:AB_2673696   Santa Cruz Biotechnology Cat#sc-615, RRID:AB_632253  

VALIDATION SUMMARY
 
 

Antibody HPA031013

  

Antibody CAB010233

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Approved  
Western blot

Uncertain   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031013

  

Antibody CAB010233

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nuclear bodies.    

Antibody dilution

 1:45    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031013

  

Antibody CAB010233

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 48 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:75  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA031013

  

Antibody CAB010233

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 105.8, 101.4, 86.6, 80.5, 75.1, 62.9   105.8, 101.4, 86.6, 80.5, 75.1, 62.9, 31.1, 27.8, 21.4, 19.9, 17.8, 7.9, 4.6  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA031013

  

Antibody CAB010233

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA031013

  

Antibody CAB010233

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 101    

Antigen sequence

 
ESVLIALENLMTTCYGPLPSTNSYENNKTDVSAADIVLSKTAETDVLFNK
VESSGKNYSNVDTSVIPFQNDMHNDESGKNTDDCLNHQISIGDFGYGHCS
S
   

Matching transcripts

 PMS1-001 - ENSP00000406490 [100%]
PMS1-003 - ENSP00000387125 [100%]
PMS1-006 - ENSP00000389938 [100%]
PMS1-010 - ENSP00000401064 [100%]
PMS1-011 - ENSP00000387169 [100%]
PMS1-201 - ENSP00000404492 [100%]
PMS1-202 - ENSP00000398378 [100%]
PMS1-204 - ENSP00000485312 [100%]
   

Other gene match

    
ANTIGEN VIEW
PMS1-001
PMS1-003
PMS1-006
PMS1-010
PMS1-011
PMS1-201
PMS1-202
PMS1-204
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.