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Antibody staining overlaps with antibody HPA054354.
Validated
Antibody staining overlaps with antibody HPA027509.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli & nuclear membrane.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Antibody dilution
1:50
1:49
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027509
Antibody HPA054354
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebellum shows strong nucleolar positivity in purkinje cells.
Expression
RNA: detected in 37 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
Antibody dilution
1:70
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027509
Antibody HPA054354
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
106.1
106.1
Antibody dilution
1:250
1:240
PROTEIN ARRAY
Antibody HPA027509
Antibody HPA054354
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:6000
1:1950
ANTIGEN INFORMATION
Antibody HPA027509
Antibody HPA054354
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
101
106
Antigen sequence
AKEDQPAVPGETQGDSYFTGKKTIETVKAAERIMEAIELYREETAKMKEH
KAICKAAGKEVPLPSNPILMAYGSISPSAYVLEIFKGIKSSELEESLLVL
P