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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Antibody dilution
1:27
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA045899
Antibody HPA071952
Standard validation
Approved
Figure description
Immunohistochemical staining of human esophagus shows strong cytoplasmic and nuclear positivity in superficial layers of squamous epithelial cells.
Expression
RNA: detected in 16 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
Antibody dilution
1:1250
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045899
Antibody HPA071952
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402694)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
98.2, 98.1, 96.1
98.2, 98.1, 96.1
Antibody dilution
1:250
1:90
PROTEIN ARRAY
Antibody HPA045899
Antibody HPA071952
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
