Expression of MTHFD2 in cancer - Summary

GENERAL INFORMATION
Gene name	MTHFD2
Gene description	Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Protein class	Enzymes Predicted intracellular proteins Predicted secreted proteins
Predicted localization	Intracellular,Secreted
Number of transcripts	3
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Expressed in all
HPA (normal tissue):	Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in several different tissues, distinct expression in immune cells.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly consistent with RNA expression data. Pending external verification.
Reliability score	Approved based on 2 antibodies. HPA049657 , CAB003684
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PROGNOSTIC SUMMARY
Prognostic marker in renal cancer (unfavourable), endometrial cancer (unfavourable) and glioma (favourable).
Renal cancer p<0.001 Endometrial cancer p<0.001 Glioma p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

STAINING SUMMARY

HPA049657	CAB003684

GENE INFORMATION
Gene name	MTHFD2
Synonyms
Description	Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (HGNC Symbol)
Entrez gene summary	This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]
Chromosome	2
Cytoband	p13.1
Chromosome location (bp)	74198562 - 74217565
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000065911 (version 83.38)
Entrez gene	10797
UniProt	P13995 (UniProt - Evidence at protein level)
neXtProt	NX_P13995
Antibodypedia	MTHFD2 antibodies

PROTEIN BROWSER

MTHFD2-001	MTHFD2-003	MTHFD2-009

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
MTHFD2-001 ENSP00000377617 ENST00000394053	P13995 [Direct mapping] Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase Methenyltetrahydrofolate cyclohydrolase Show all	Enzymes ENZYME proteins Oxidoreductases Hydrolases SPOCTOPUS predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000287 [magnesium ion binding] GO:0003824 [catalytic activity] GO:0004477 [methenyltetrahydrofolate cyclohydrolase activity] GO:0004487 [methylenetetrahydrofolate dehydrogenase (NAD+) activity] GO:0004488 [methylenetetrahydrofolate dehydrogenase (NADP+) activity] GO:0005615 [extracellular space] GO:0005739 [mitochondrion] GO:0006730 [one-carbon metabolic process] GO:0009396 [folic acid-containing compound biosynthetic process] GO:0042301 [phosphate ion binding] GO:0046653 [tetrahydrofolate metabolic process] GO:0055114 [oxidation-reduction process] Show all	350 aa 37.9 kDa	Yes	0
MTHFD2-003 ENSP00000386536 ENST00000409804	B9A062 [Direct mapping] Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial Show all	SPOCTOPUS predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003824 [catalytic activity] GO:0004477 [methenyltetrahydrofolate cyclohydrolase activity] GO:0004488 [methylenetetrahydrofolate dehydrogenase (NADP+) activity] GO:0005739 [mitochondrion] GO:0006730 [one-carbon metabolic process] GO:0009396 [folic acid-containing compound biosynthetic process] GO:0055114 [oxidation-reduction process] Show all	222 aa 23.9 kDa	Yes	0
MTHFD2-009 ENSP00000386542 ENST00000409601	B8ZZU9 [Direct mapping] Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial Show all	SPOCTOPUS predicted membrane proteins Phobius predicted secreted proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003824 [catalytic activity] GO:0004477 [methenyltetrahydrofolate cyclohydrolase activity] GO:0004488 [methylenetetrahydrofolate dehydrogenase (NADP+) activity] GO:0005739 [mitochondrion] GO:0006730 [one-carbon metabolic process] GO:0009396 [folic acid-containing compound biosynthetic process] GO:0055114 [oxidation-reduction process] Show all	267 aa 29 kDa	No	0