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Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Expression
RNA: detected in 31 tissues Protein: detected in 17 cell types
RNA: detected in 31 tissues Protein: detected in 17 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:250
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036493
Antibody HPA036669
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418163)
Target mass (kDa)
92.9, 91.9
92.9, 91.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA036493
Antibody HPA036669
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.