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ROCK1

ANTIBODY INFORMATION
 
 

Antibody HPA007567

  

Antibody HPA045639

  

Antibody CAB004562

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA007567   HPA045639   sc-17794  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 4   10   2  

References

      

Proper citation

 Atlas Antibodies Cat#HPA007567, RRID:AB_1856393   Atlas Antibodies Cat#HPA045639, RRID:AB_2679401   Santa Cruz Biotechnology Cat#sc-17794, RRID:AB_628223  

VALIDATION SUMMARY
 
 

Antibody HPA007567

  

Antibody HPA045639

  

Antibody CAB004562

  
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007567

  

Antibody HPA045639

  

Antibody CAB004562

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.   Immunohistochemical staining of human bone marrow shows distinct positivity in megakaryocytes.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 69 cell types		   RNA: detected in 35 tissues
Protein: detected in 70 cell types		   RNA: detected in 35 tissues
Protein: detected in 24 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:15   1:35  

Literature conformity

 Partly consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007567

  

Antibody HPA045639

  

Antibody CAB004562

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 158.2   158.2, 11.3   158.2, 11.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA007567

  

Antibody HPA045639

  

Antibody CAB004562

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA007567

  

Antibody HPA045639

  

Antibody CAB004562

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 140   30    

Antigen sequence

 
TQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAESEQLARG
LLEEQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRR
ENEELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERT
  
KEDLICPCKVSYDVTSARDMLLLACSQDEQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					ROCK1-001 - ENSP00000382697 [100%] 

											 
																						ROCK1-001 - ENSP00000382697 [100%] 
ROCK1-003 - ENSP00000462004 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ROCK1-001
ROCK1-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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