Fields »
ABOUT
HELP
BLOG
POLR1A

ANTIBODY INFORMATION
 
 

Antibody HPA031513

  

Antibody HPA049700

  

Antibody CAB010145

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA031513   HPA049700   sc-48385  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   12   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA031513, RRID:AB_2673916   Atlas Antibodies Cat#HPA049700, RRID:AB_2680856   Santa Cruz Biotechnology Cat#sc-48385, RRID:AB_675814  

VALIDATION SUMMARY
 
 

Antibody HPA031513

  

Antibody HPA049700

  

Antibody CAB010145

  
Immunocytochemistry

  Approved   Approved  
Immunohistochemistry

Supported     Supported  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031513

  

Antibody HPA049700

  

Antibody CAB010145

  

Standard validation

   Approved   Approved  

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center.  

Antibody dilution

   1:74   1:25  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031513

  

Antibody HPA049700

  

Antibody CAB010145

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.     Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		     RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:350     1:100  

Literature conformity

 Consistent with gene/protein characterization data.     Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA031513

  

Antibody HPA049700

  

Antibody CAB010145

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 194.8, 187.8   194.8, 187.8   194.8, 187.8  

Antibody dilution

 1:250   1:370   1:500  

PROTEIN ARRAY
 
 

Antibody HPA031513

  

Antibody HPA049700

  

Antibody CAB010145

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2950    

ANTIGEN INFORMATION
 
 

Antibody HPA031513

  

Antibody HPA049700

  

Antibody CAB010145

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 95   85    

Antigen sequence

 
SNYEVIMKSQHLHEVLSRADPKKALHHFRAIKKWQSKHPNTLLRRGAFLS
YSQKIQEAVKALKLESENRNGRSPGTQEMLRMWYELDEESRRKYQ
  
DEVRGKWQDAHLGKDQRDFNMIDLKFKEEVNHYSNEINKACMPFGLHRQF
PENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGR
  

											 
																				

																		

										
											
Matching transcripts

												
																					POLR1A-001 - ENSP00000263857 [100%] 
POLR1A-002 - ENSP00000386300 [100%] 

											 
																						POLR1A-001 - ENSP00000263857 [100%] 
POLR1A-002 - ENSP00000386300 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
POLR1A-001
POLR1A-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.