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GUCY2C

ANTIBODY INFORMATION
 
 

Antibody HPA037655

  

Antibody HPA073759

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037655   HPA073759  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   16  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA037655, RRID:AB_10672388    

VALIDATION SUMMARY
 
 

Antibody HPA037655

  

Antibody HPA073759

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry

Uncertain    
Western blot

Uncertain    
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037655

  

Antibody HPA073759

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.  

Antibody dilution

   1:97  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037655

  

Antibody HPA073759

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 18 tissues
Protein: detected in 60 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:550    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA037655

  

Antibody HPA073759

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		    

   

Figure description

    

Target mass (kDa)

 123.4    

Antibody dilution

 1:250    

PROTEIN ARRAY
 
 

Antibody HPA037655

  

Antibody HPA073759

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3850  

ANTIGEN INFORMATION
 
 

Antibody HPA037655

  

Antibody HPA073759

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 86   72  

Antigen sequence

 
EVRGETYLKGRGNETTYWLTGMKDQKFNLPTPPTVENQQRLQAEFSDMIA
NSLQKRQAAGIRSQKPRRVASYKKGTLEYLQLNTTD
  
KWSHIPPENIFPLETNETNHVSLKIDDDKRRDTIQRLRQCKYDKKRVILK
DLKHNDGNFTEKQKIELNKLLQ
 

Matching transcripts

 GUCY2C-001 - ENSP00000261170 [100%]
  GUCY2C-001 - ENSP00000261170 [100%]
 

Other gene match

    
ANTIGEN VIEW
GUCY2C-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA037655

  

Antibody HPA073759

  

1.

 The guanylate cyclase-C signaling pathway is down-regulated in inflammatory bowel disease
Brenna Ø et al
Scand J Gastroenterol 2015;50(10):1241-52
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.