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Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity with a granular pattern in cells of tubules.
Expression
RNA: detected in 5 tissues Protein: detected in 46 cell types
RNA: detected in 5 tissues Protein: detected in 53 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:10
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042115
Antibody HPA043315
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415198)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415198)
Target mass (kDa)
132.3, 131.5, 131.4, 130.8
132.3, 131.5, 131.4, 130.8
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA042115
Antibody HPA043315
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
