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EXOSC7

ANTIBODY INFORMATION
 
 

Antibody HPA036182

  

Antibody HPA057980

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036182   HPA057980  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA036182, RRID:AB_10673346   Atlas Antibodies Cat#HPA057980, RRID:AB_2683571  

VALIDATION SUMMARY
 
 

Antibody HPA036182

  

Antibody HPA057980

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036182

  

Antibody HPA057980

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.  

Antibody dilution

   1:111  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036182

  

Antibody HPA057980

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human rectum shows distinct nucleolar positivity in glandular cells.   Immunohistochemical staining of human skin shows moderate nucleolar positivity in epidermal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 61 cell types		   RNA: detected in 37 tissues
Protein: detected in 20 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:1800  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036182

  

Antibody HPA057980

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 31.8   31.8  

Antibody dilution

 1:250   1:370  

PROTEIN ARRAY
 
 

Antibody HPA036182

  

Antibody HPA057980

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:4450  

ANTIGEN INFORMATION
 
 

Antibody HPA036182

  

Antibody HPA057980

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 94   93  

Antigen sequence

 
PCIVTLCKIGYRHVVDATLQEEACSLASLLVSVTSKGVVTCMRKVGKGSL
DPESIFEMMETGKRVGKVLHASLQSVLHKEESLGPKRQKVGFLG
  
EDLRVDGRGCEDYRCVEVETDVVSNTSGSARVKLGHTDILVGVKAEMGTP
KLEKPNEGYLEFFVDCSASATPEFEGRGGDDLGTEIANTLYRI
 

Matching transcripts

 EXOSC7-001 - ENSP00000265564 [99%]
  EXOSC7-001 - ENSP00000265564 [100%]
 

Other gene match

    
ANTIGEN VIEW
EXOSC7-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.