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Antibody staining overlaps with GFP tagged protein
Validated
Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus but excluded from the nucleoli and GFP expression in nucleus but excluded from the nucleoli.
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus but excluded from the nucleoli and GFP expression in nucleus but excluded from the nucleoli.
Antibody dilution
1:39
1:4
Formal validation: Independent
Validated
Antibody staining overlaps with antibody HPA060714.
Validated
Antibody staining overlaps with antibody HPA052707.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
Antibody dilution
1:39
1:4
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA052707
Antibody HPA060714
Antibody CAB013294
Antibody CAB070868
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.
Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 27 cell types
RNA: detected in 37 tissues Protein: detected in 61 cell types
RNA: detected in 37 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:25
1:200
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052707
Antibody HPA060714
Antibody CAB013294
Antibody CAB070868
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
