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NMRK2

ANTIBODY INFORMATION
 
 

Antibody HPA049909

  

Antibody HPA054792

  

Antibody HPA072450

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049909   HPA054792   HPA072450  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 10   16   16  

References

      

Proper citation

   Atlas Antibodies Cat#HPA054792, RRID:AB_2682601   Atlas Antibodies Cat#HPA072450, RRID:AB_2686522  

VALIDATION SUMMARY
 
 

Antibody HPA049909

  

Antibody HPA054792

  

Antibody HPA072450

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

    Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049909

  

Antibody HPA054792

  

Antibody HPA072450

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA054792.		  
Validated

Antibody staining overlaps with antibody HPA049909.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & vesicles.   Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & vesicles.    

Antibody dilution

 1:9   1:200    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049909

  

Antibody HPA054792

  

Antibody HPA072450

  

Standard validation

     Approved  

     

Figure description

     Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.  

Expression

     RNA: detected in 5 tissues
Protein: detected in 7 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:500  

Literature conformity

     Partly consistent with gene/protein characterization data.  

RNA consistency

     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA049909

  

Antibody HPA054792

  

Antibody HPA072450

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 26.6, 26, 12.4   26.6, 26   26.6, 26  

Antibody dilution

 1:90   1:1000   1:120  

PROTEIN ARRAY
 
 

Antibody HPA049909

  

Antibody HPA054792

  

Antibody HPA072450

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:700   1:8000   1:1300  

ANTIGEN INFORMATION
 
 

Antibody HPA049909

  

Antibody HPA054792

  

Antibody HPA072450

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 76   63   63  

Antigen sequence

 
KPQDQIAVGEDGFKQWDVLESLDMEAMLDTVQAWLSSPQKFARAHGVSVQ
PEASDTHILLLEGFLLYSYKPLVDLY
  
VYLDGMKSREELFREVLEDIQNSLLNRSQESAPSPARPARTQGPGRGCGH
RTARPAASQQDSM
  
VYLDGMKSREELFREVLEDIQNSLLNRSQESAPSPARPARTQGPGRGCGH
RTARPAASQQDSM
 

Matching transcripts

 NMRK2-001 - ENSP00000168977 [100%]
NMRK2-002 - ENSP00000472581 [99%]
NMRK2-201 - ENSP00000480091 [99%]
NMRK2-003 - ENSP00000471436 [91%]
  NMRK2-001 - ENSP00000168977 [100%]
NMRK2-002 - ENSP00000472581 [100%]
NMRK2-201 - ENSP00000480091 [100%]
  NMRK2-001 - ENSP00000168977 [100%]
NMRK2-002 - ENSP00000472581 [100%]
NMRK2-201 - ENSP00000480091 [100%]
 

Other gene match

      
ANTIGEN VIEW
NMRK2-001
NMRK2-002
NMRK2-003
NMRK2-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.