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ANTIBODY INFORMATION
 
 

Antibody HPA001612

  

Antibody HPA001613

  

Antibody CAB004393

  

Antibody CAB068187

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001612   HPA001613   sc-25281   AMAb90696  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 2   3   2   13  

References

 2   3      

Proper citation

 Atlas Antibodies Cat#HPA001612, RRID:AB_1078825   Atlas Antibodies Cat#HPA001613, RRID:AB_1078824   Santa Cruz Biotechnology Cat#sc-25281, RRID:AB_671972   Atlas Antibodies Cat#AMAb90696, RRID:AB_2665634  

VALIDATION SUMMARY
 
 

Antibody HPA001612

  

Antibody HPA001613

  

Antibody CAB004393

  

Antibody CAB068187

  
Immunocytochemistry

      Approved  
Immunohistochemistry


Validated
  

Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Uncertain   Supported  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001612

  

Antibody HPA001613

  

Antibody CAB004393

  

Antibody CAB068187

  

Standard validation

       Approved  

       

Figure description

       Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.  

Antibody dilution

       1:25  

Literature conformity

       The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001612

  

Antibody HPA001613

  

Antibody CAB004393

  

Antibody CAB068187

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 
 
   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human cervix, uterine shows strong cytoplasmic positivity in glandular cells and extracellular matrix.   Immunohistochemical staining of human cervix, uterine shows moderate positivity in extracellular matrix.   Immunohistochemical staining of human cervix, uterine shows moderate cytoplasmic positivity in glandular cells and extracellular matrix.   Immunohistochemical staining of human cervix, uterine shows moderate cytopalsmic positivity in glandular cells and extracellular matrix.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 52 cell types		   RNA: detected in 37 tissues
Protein: detected in 13 cell types		   RNA: detected in 37 tissues
Protein: detected in 35 cell types		   RNA: detected in 37 tissues
Protein: detected in 26 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:350   1:50   1:6000   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001612

  

Antibody HPA001613

  

Antibody CAB004393

  

Antibody CAB068187

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416606)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416606)		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 78.3, 77.2, 74.4, 65.4, 61.6   78.3, 77.2, 74.4, 65.4, 61.6   78.3, 77.2, 74.4, 65.4, 61.6, 20.5, 19.4, 18.4, 15.4, 7.2, 6.9   78.3, 77.2, 74.4, 65.4, 61.6, 20.5, 19.4, 18.4, 15.4, 7.2, 6.9  

Antibody dilution

 1:250   1:250   1:500   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA001612

  

Antibody HPA001613

  

Antibody CAB004393

  

Antibody CAB068187

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA001612

  

Antibody HPA001613

  

Antibody CAB004393

  

Antibody CAB068187

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 137   143      

Antigen sequence

 
CESGIHNCLPDFICQNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSI
SAPCPIGHTCINTEGSYTCQKNVPNCGRGYHLNEEGTRCVDVDECAPPAE
PCGKGHRCVNSPGSFRCECKTGYYFDGISRMCVDVNE
  
NTLGSYLCSCSVGFRLSVDGRSCEDINECSSSPCSQECANVYGSYQCYCR
RGYQLSDVDGVTCEDIDECALPTGGHICSYRCINIPGSFQCSCPSSGYRL
APNGRNCQDIDECVTGIHNCSINETCFNIQGGFRCLAFECPEN
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					FBLN1-001 - ENSP00000331544 [100%] 
FBLN1-002 - ENSP00000385521 [100%] 
FBLN1-003 - ENSP00000262722 [100%] 
FBLN1-004 - ENSP00000393812 [100%] 
FBLN1-019 - ENSP00000342212 [100%] 

											 
																						FBLN1-001 - ENSP00000331544 [100%] 
FBLN1-002 - ENSP00000385521 [100%] 
FBLN1-003 - ENSP00000262722 [100%] 
FBLN1-004 - ENSP00000393812 [100%] 
FBLN1-019 - ENSP00000342212 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FBLN1-001
FBLN1-002
FBLN1-003
FBLN1-004
FBLN1-019
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001612

												
										 
																				
											
											
											
Antibody HPA001613

												
										 
																				
											
											
											
Antibody CAB004393

												
										 
																				
											
											
											
Antibody CAB068187

												
										 
																		

																	

										
											
1.

												
																						Identification of a progenitor cell of origin capable of generating diverse meningioma histological subtypes

	Kalamarides M et al

	Oncogene 2011;30(20):2333-44

			
											 
																							Plasma profiling reveals human fibulin-1 as candidate marker for renal impairment

	Neiman M et al

	J Proteome Res 2011;10(11):4925-34

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Plasma profiling reveals human fibulin-1 as candidate marker for renal impairment

	Neiman M et al

	J Proteome Res 2011;10(11):4925-34

			
											 
																							Promoter hypermethylation of fibulin 1 gene is associated with tumor progression in hepatocellular carcinoma

	Kanda M et al

	Mol Carcinog 2011;50(8):571-9

			
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																					
											 
																							Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria

	Bachmann J et al

	PLoS Pathog 2014;10(4):e1004038

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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