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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:7
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA006567
Antibody CAB017700
Standard validation
Supported
Figure description
Immunohistochemical staining of human soft tissue shows strong cytoplasmic positivity in adipocytes.
Expression
RNA: detected in 36 tissues Protein: detected in 26 cell types
Retrieval
HIER pH6
Antibody dilution
1:1000
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA006567
Antibody CAB017700
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
116.6, 28.7, 24.7, 19.6
116.6, 32.6, 29.2, 28.7, 24.7, 19.6, 8.6
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA006567
Antibody CAB017700
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
