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Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus & cell junctions.
Antibody dilution
1:102
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA030760
Antibody HPA067496
Antibody CAB006844
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity with a granular pattern in neuronal cells.
Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
RNA: detected in 37 tissues Protein: detected in 42 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:35
1:500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030760
Antibody HPA067496
Antibody CAB006844
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
