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TCF7

ANTIBODY INFORMATION
 
 

Antibody HPA058863

  

Antibody HPA070505

  

Antibody CAB019403

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Cell Signaling Technology, Inc
  

Product name

 HPA058863   HPA070505   2203  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 16   16   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA058863, RRID:AB_2683837   Atlas Antibodies Cat#HPA070505, RRID:AB_2686275   Cell Signaling Technology Cat#2203, RRID:AB_2199302  

VALIDATION SUMMARY
 
 

Antibody HPA058863

  

Antibody HPA070505

  

Antibody CAB019403

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

   

Validated
  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA058863

  

Antibody HPA070505

  

Antibody CAB019403

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.    

Antibody dilution

 1:61   1:95    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA058863

  

Antibody HPA070505

  

Antibody CAB019403

  

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

     Supported  

     

Figure description

     Immunohistochemical staining of human tonsil shows strong nuclear positivity in lymphoid cells outside reaction centra.  

Expression

     RNA: detected in 36 tissues
Protein: detected in 16 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:50  

Literature conformity

     Consistent with extensive gene/protein characterization data.  

RNA consistency

     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA058863

  

Antibody HPA070505

  

Antibody CAB019403

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 52   52, 41.6, 30.3, 30.2, 16, 15.7   52, 41.6, 30.3, 30.2, 22.7, 18.2, 16.8, 16.5, 16, 15.7, 13.3, 12.6  

Antibody dilution

 1:200   1:320   1:500  

PROTEIN ARRAY
 
 

Antibody HPA058863

  

Antibody HPA070505

  

Antibody CAB019403

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2450   1:3800    

ANTIGEN INFORMATION
 
 

Antibody HPA058863

  

Antibody HPA070505

  

Antibody CAB019403

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 54   85    

Antigen sequence

 
KKCIRYLPGEGRCPSPVPSDDSALGCPGSPAPQDSPSYHLLPRFPTELLT
SPAE
  
KQVHRPLQTPDLSGFYSLTSGSMGQLPHTVSWFTHPSLMLGSGVPGHPAA
IPHPAIVPPSGKQELQPFDRNLKTQAESKAEKEAK
  

											 
																				

																		

										
											
Matching transcripts

												
																					TCF7-002 - ENSP00000378472 [100%] 

											 
																						TCF7-001 - ENSP00000340347 [100%] 
TCF7-002 - ENSP00000378472 [100%] 
TCF7-003 - ENSP00000378469 [100%] 
TCF7-004 - ENSP00000367822 [100%] 
TCF7-006 - ENSP00000430179 [100%] 
TCF7-014 - ENSP00000429696 [100%] 
TCF7-024 - ENSP00000429547 [100%] 
TCF7-012 - ENSP00000427782 [96%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TCF7-001
TCF7-002
TCF7-003
TCF7-004
TCF7-006
TCF7-012
TCF7-014
TCF7-024
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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