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PKP1

ANTIBODY INFORMATION
 
 

Antibody HPA027221

  

Antibody HPA027589

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA027221   HPA027589  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   6  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA027221, RRID:AB_10599737   Atlas Antibodies Cat#HPA027589, RRID:AB_10611387  

VALIDATION SUMMARY
 
 

Antibody HPA027221

  

Antibody HPA027589

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA027221

  

Antibody HPA027589

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & plasma membrane.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:88   1:62  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027221

  

Antibody HPA027589

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA027589 across 75 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA027221 across 75 cell types.		  
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human oral mucosa shows strong cytoplasmic, membranous and nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human pancreas shows strong positivity in a subset of exocrine glandular cells.  

Expression

 RNA: detected in 15 tissues
Protein: detected in 68 cell types		   RNA: detected in 15 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:20  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA027221

  

Antibody HPA027589

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 82.9, 80.5   82.9, 80.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA027221

  

Antibody HPA027589

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA027221

  

Antibody HPA027589

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 85   98  

Antigen sequence

 
YTVRNLMASQPQLAKQYFSSSMLNNIINLCRSSASPKAAEAARLLLSDMW
SSKELQGVLRQQGFDRNMLGTLAGANSLRNFTSRF
  
RFSSYSQMENWSRHYPRGSCNTTGAGSDICFMQKIKASRSEPDLYCDPRG
TLRKGTLGSKGQKTTQNRYSFYSTCSGQKAIKKCPVRPPSCASKQDPV
 

Matching transcripts

 PKP1-001 - ENSP00000356293 [100%]
PKP1-004 - ENSP00000295597 [100%]
PKP1-201 - ENSP00000263946 [100%]
PKP1-202 - ENSP00000482213 [100%]
  PKP1-001 - ENSP00000356293 [100%]
PKP1-004 - ENSP00000295597 [100%]
PKP1-201 - ENSP00000263946 [100%]
PKP1-202 - ENSP00000482213 [100%]
 

Other gene match

    
ANTIGEN VIEW
PKP1-001
PKP1-004
PKP1-201
PKP1-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA027221

  

Antibody HPA027589

  

1.

 Aberrantly methylated PKP1 in the progression of Barrett's esophagus to esophageal adenocarcinoma
Kaz AM et al
Genes Chromosomes Cancer 2012;51(4):384-93
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.