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ATP8B1

ANTIBODY INFORMATION
 
 

Antibody HPA018673

  

Antibody HPA018674

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA018673   HPA018674  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

    

Proper citation

 Atlas Antibodies Cat#HPA018673, RRID:AB_1845207   Atlas Antibodies Cat#HPA018674, RRID:AB_1845208  

VALIDATION SUMMARY
 
 

Antibody HPA018673

  

Antibody HPA018674

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018673

  

Antibody HPA018674

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 68 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:20  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018673

  

Antibody HPA018674

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 143.7   143.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA018673

  

Antibody HPA018674

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA018673

  

Antibody HPA018674

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 108   75  

Antigen sequence

 
TTFDEDSQPNDEVVPYSDDETEDELDDQGSAVEPEQNRVNREAEENREPF
RKECTWQVKANDRKYHEQPHFMNTKFLCIKESKYANNAIKTYKYNAFTFI
PMNLFEQF
  
KIWVLTGDKKETAENIGFACELLTEDTTICYGEDINSLLHARMENQRNRG
GVYAKFAPPVQESFFPPGGNRALII
 

Matching transcripts

 ATP8B1-001 - ENSP00000283684 [100%]
ATP8B1-201 - ENSP00000445359 [100%]
  ATP8B1-001 - ENSP00000283684 [100%]
ATP8B1-201 - ENSP00000445359 [100%]
 

Other gene match

    
ANTIGEN VIEW
ATP8B1-001
ATP8B1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.