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B4GALT1

ANTIBODY INFORMATION
 
 

Antibody HPA010806

  

Antibody HPA010807

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA010806   HPA010807  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   3  

References

   1  

Proper citation

 Atlas Antibodies Cat#HPA010806, RRID:AB_1078255   Atlas Antibodies Cat#HPA010807, RRID:AB_1078254  

VALIDATION SUMMARY
 
 

Antibody HPA010806

  

Antibody HPA010807

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA010806

  

Antibody HPA010807

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA010807.		  
Validated

Antibody staining overlaps with antibody HPA010806.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line HeLa shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.  

Antibody dilution

 1:4   1:10  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA010806

  

Antibody HPA010807

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human lateral ventricle shows strong granular cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 43 cell types		   RNA: detected in 37 tissues
Protein: detected in 53 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA010806

  

Antibody HPA010807

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 43.9   43.9, 24.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA010806

  

Antibody HPA010807

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA010806

  

Antibody HPA010807

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 76   114  

Antigen sequence

 
FRGMSISRPNAVVGRCRMIRHSRDKKNEPNPQRFDRIAHTKETMLSDGLN
SLTYQVLDVQRYPLYTQITVDIGTPS
  
LPQLVGVSTPLQGGSNSAAAIGQSSGELRTGGARPPPPLGASSQPRPGGD
SSPVVDSGPGPASNLTSVPVPHTTALSLPACPEESPLLVGPMLIEFNMPV
DLELVAKQNPNVKM
 

Matching transcripts

 B4GALT1-001 - ENSP00000369055 [100%]
  B4GALT1-001 - ENSP00000369055 [100%]
B4GALT1-201 - ENSP00000440341 [100%]
 

Other gene match

    
ANTIGEN VIEW
B4GALT1-001
B4GALT1-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA010806

  

Antibody HPA010807

  

1.

   The rough endoplasmatic reticulum is a central nucleation site of siRNA-mediated RNA silencing
Stalder L et al
EMBO J 2013;32(8):1115-27
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.