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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts.
Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity of cells in seminiferus ducts.
Expression
RNA: detected in 2 tissues Protein: detected in 63 cell types
RNA: detected in 2 tissues Protein: detected in 21 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:450
1:3000
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019259
Antibody CAB016416
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413869)
Target mass (kDa)
67.3
67.3, 16.8, 12.9
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA019259
Antibody CAB016416
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.