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TFAP2C

ANTIBODY INFORMATION
 
 

Antibody HPA055179

  

Antibody HPA057076

  

Antibody CAB016061

  

Antibody CAB016079

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Santa Cruz Biotechnology
  

Product name

 HPA055179   HPA057076   sc-12762   sc-53162  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 14   14   4   4  

References

        

Proper citation

 Atlas Antibodies Cat#HPA055179, RRID:AB_2682729   Atlas Antibodies Cat#HPA057076, RRID:AB_2683329   Santa Cruz Biotechnology Cat#sc-12762, RRID:AB_667770   Santa Cruz Biotechnology Cat#sc-53162, RRID:AB_2199447  

VALIDATION SUMMARY
 
 

Antibody HPA055179

  

Antibody HPA057076

  

Antibody CAB016061

  

Antibody CAB016079

  
Immunocytochemistry

Supported   Supported      
Immunohistochemistry


Validated
    

Validated
  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA055179

  

Antibody HPA057076

  

Antibody CAB016061

  

Antibody CAB016079

  

Standard validation

 Supported   Supported      

       

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.      

Antibody dilution

 1:93   1:34      

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA055179

  

Antibody HPA057076

  

Antibody CAB016061

  

Antibody CAB016079

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported     Supported   Supported  

       

Figure description

 Immunohistochemical staining of human skin shows moderate nuclear positivity in epidermal cells.     Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.   Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.  

Expression

 RNA: detected in 21 tissues
Protein: detected in 31 cell types		     RNA: detected in 21 tissues
Protein: detected in 32 cell types		   RNA: detected in 21 tissues
Protein: detected in 20 cell types		  

Retrieval

 HIER pH6     HIER pH6   HIER pH6  

Antibody dilution

 1:1000     1:50   1:50  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA055179

  

Antibody HPA057076

  

Antibody CAB016061

  

Antibody CAB016079

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		        

Target mass (kDa)

 49.2   49.2   49.2, 12.6   49.2, 12.6  

Antibody dilution

 1:470   1:110   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA055179

  

Antibody HPA057076

  

Antibody CAB016061

  

Antibody CAB016079

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3750   1:1200      

ANTIGEN INFORMATION
 
 

Antibody HPA055179

  

Antibody HPA057076

  

Antibody CAB016061

  

Antibody CAB016079

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Not known  

Length (aa)

 78   37      

Antigen sequence

 
RSDLLLPHAHALDAAGLAENLGLHDMPHQMDEVQNVDDQHLLLHDQTVIR
KGPISMTKNPLNLPCQKELVGAVMNPTE
  
NYIKEALIVIDKSYMNPGDQSPADSNKTLEKMEKHRK
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					TFAP2C-001 - ENSP00000201031 [100%] 

											 
																						TFAP2C-001 - ENSP00000201031 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TFAP2C-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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