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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Antibody dilution
1:5
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA047083
Antibody HPA050396
Standard validation
Supported
Figure description
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells and islets of Langerhans.
Expression
RNA: detected in 37 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047083
Antibody HPA050396
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
98.8
98.8, 52.4
Antibody dilution
1:200
1:80
PROTEIN ARRAY
Antibody HPA047083
Antibody HPA050396
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
