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Cytoplasmic region of segmented cells in 10x-images
Antibody dilution
1:49
Standard validation
Approved
Approved
Approved
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli, plasma membrane & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:100
1:50
1:20
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA029761
Antibody HPA029762
Antibody HPA029763
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA029763 across 46 cell lines. Pearson correlation with HPA029762 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA029763 across 46 cell lines. Pearson correlation with HPA029761 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA029762 across 46 cell lines. Pearson correlation with HPA029761 across 46 cell lines.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in reaction center cells.
Expression
RNA: detected in 37 tissues Protein: detected in 60 cell types
RNA: detected in 37 tissues Protein: detected in 18 cell types
RNA: detected in 37 tissues Protein: detected in 47 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:300
1:50
1:125
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029761
Antibody HPA029762
Antibody HPA029763
Formal validation: Genetic
Validated
Downregulation visible in both siRNA lanes
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: siRNA 1 Lane 3: siRNA 2 Lane 4: Scrambled
