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SLC17A6

ANTIBODY INFORMATION
 
 

Antibody HPA039226

  

Antibody CAB037130

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA039226   V2514  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   7  

References

    

Proper citation

 Atlas Antibodies Cat#HPA039226, RRID:AB_2676400   Sigma-Aldrich Cat#V2514, RRID:AB_477611  

VALIDATION SUMMARY
 
 

Antibody HPA039226

  

Antibody CAB037130

  
Immunohistochemistry


Validated
   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039226

  

Antibody CAB037130

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human bronchus shows strong membranous positivity in respiratory epithelial cells.   Immunohistochemical staining of human lateral ventricle shows moderate cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 28 cell types		   RNA: detected in 1 tissues
Protein: detected in 17 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:2200   1:2000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  
 

Standard validation

 Supported    

   

Figure description

 Wide expression in neurons.    

WESTERN BLOT
 
 

Antibody HPA039226

  

Antibody CAB037130

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 64.4   64.4  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA039226

  

Antibody CAB037130

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:6000    

ANTIGEN INFORMATION
 
 

Antibody HPA039226

  

Antibody CAB037130

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 66    

Antigen sequence

 
CGFIHEDELDEETGDITQNYINYGTTKSYGATTQANGGWPSGWEKKEEFV
QGEVQDSHSYKDRVDY
  

											 
																				

																		

										
											
Matching transcripts

												
																					SLC17A6-001 - ENSP00000263160 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SLC17A6-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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