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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & vesicles.
Antibody dilution
1:100
1:50
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA017880
Antibody HPA027551
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human urinary bladder shows nuclear positivity in urothelial cells.
Immunohistochemical staining of human gallbladder shows moderate nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 59 cell types
RNA: detected in 37 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:125
1:30
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017880
Antibody HPA027551
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415032)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415032)
Target mass (kDa)
66.2
66.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA017880
Antibody HPA027551
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
