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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli, cytosol & vesicles.
Immunofluorescent staining of human cell line HeLa shows localization to vesicles.
Antibody dilution
1:115
1:49
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA047844
Antibody HPA060975
Standard validation
Approved
Figure description
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity.
Expression
RNA: detected in 37 tissues Protein: detected in 76 cell types
Retrieval
HIER pH6
Antibody dilution
1:300
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047844
Antibody HPA060975
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
