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Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic and nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Expression
RNA: detected in 11 tissues Protein: detected in 23 cell types
RNA: detected in 11 tissues Protein: detected in 26 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:400
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003548
Antibody CAB017633
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403438)
Target mass (kDa)
40.7, 40.1, 33.6
40.7, 40.1, 33.6, 20.2
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA003548
Antibody CAB017633
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.