We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Antibody staining overlaps with antibody HPA047114. Antibody staining overlaps with antibody HPA046412.
Validated
Antibody staining overlaps with antibody HPA047114. Antibody staining overlaps with antibody HPA043233.
Validated
Antibody staining overlaps with antibody HPA046412. Antibody staining overlaps with antibody HPA043233.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:141
1:31
1:38
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA043233
Antibody HPA046412
Antibody HPA047114
Antibody HPA047116
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA047116 across 46 cell lines. Pearson correlation with HPA047114 across 46 cell lines. Pearson correlation with HPA046412 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA047116 across 46 cell lines. Pearson correlation with HPA047114 across 46 cell lines. Pearson correlation with HPA043233 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA047116 across 46 cell lines. Pearson correlation with HPA046412 across 46 cell lines. Pearson correlation with HPA043233 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA047114 across 46 cell lines. Pearson correlation with HPA046412 across 46 cell lines. Pearson correlation with HPA043233 across 46 cell lines.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic and nuclear positivity in islets of Langerhans.
Immunohistochemical staining of human urinary bladder shows moderate nuclear, cytoplasmic and membranous positivity in urothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 74 cell types
RNA: detected in 37 tissues Protein: detected in 77 cell types
RNA: detected in 37 tissues Protein: detected in 68 cell types
RNA: detected in 37 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:3000
1:1000
1:1200
1:800
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043233
Antibody HPA046412
Antibody HPA047114
Antibody HPA047116
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
41.3, 23.9
41.3, 28.2
41.3, 28.2, 23.9, 18.1
41.3, 28.2
Antibody dilution
1:250
1:250
1:190
1:170
PROTEIN ARRAY
Antibody HPA043233
Antibody HPA046412
Antibody HPA047114
Antibody HPA047116
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.