ACO2 - Antibodies - The Human Protein Atlas

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ACO2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA001097

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA001097

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA001097, RRID:AB_1078092

VALIDATION SUMMARY

Antibody HPA001097

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA001097

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

Antibody dilution

1:20

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA001097

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 69 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA001097

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

87.8, 85.4

Antibody dilution

1:500

PROTEIN ARRAY

Antibody HPA001097

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA001097

Antigen

Recombinant protein fragment

Length (aa)

150

Antigen sequence

GKKFRLEAPDADELPKGEFDPGQDTYQHPPKDSSGQHVDVSPTSQRLQLL
EPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLL
IGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEG

Matching transcripts

ACO2-001 - ENSP00000216254 [100%]
ACO2-002 - ENSP00000379769 [100%]

Other gene match

ANTIGEN VIEW