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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in a subset of neuronal cells.
Expression
RNA: detected in 14 tissues Protein: detected in 31 cell types
RNA: detected in 14 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1200
1:200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003040
Antibody HPA067533
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408351)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408351)
Target mass (kDa)
41.1, 24.3
41.1
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA003040
Antibody HPA067533
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
