Fields »
ABOUT
HELP
BLOG
CEP128

ANTIBODY INFORMATION
 
 

Antibody HPA000602

  

Antibody HPA001116

  

Antibody HPA018498

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000602   HPA001116   HPA018498  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 12   2   4  

References

   1    

Proper citation

   Atlas Antibodies Cat#HPA001116, RRID:AB_1078323   Atlas Antibodies Cat#HPA018498, RRID:AB_1845560  

VALIDATION SUMMARY
 
 

Antibody HPA000602

  

Antibody HPA001116

  

Antibody HPA018498

  
Immunocytochemistry

Uncertain      
Immunohistochemistry

  Uncertain   Uncertain  
Western blot

Uncertain   Supported   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000602

  

Antibody HPA001116

  

Antibody HPA018498

  

Standard validation

 Uncertain      

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol & microtubule organizing center.      

Antibody dilution

 1:50      

Literature conformity

 The subcellular location is not consistent with literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000602

  

Antibody HPA001116

  

Antibody HPA018498

  

Standard validation

   Uncertain   Uncertain  

     

Figure description

   Immunohistochemical staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human hippocampus shows moderate positivity in neuronal cells.  

Expression

   RNA: detected in 31 tissues
Protein: detected in 30 cell types		   RNA: detected in 31 tissues
Protein: detected in 65 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:500   1:750  

Literature conformity

   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000602

  

Antibody HPA001116

  

Antibody HPA018498

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

   Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 128, 18.3   128, 18.3   128  

Antibody dilution

 1:500   1:500   1:250  

PROTEIN ARRAY
 
 

Antibody HPA000602

  

Antibody HPA001116

  

Antibody HPA018498

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA000602

  

Antibody HPA001116

  

Antibody HPA018498

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 143   143   140  

Antigen sequence

 
EEMGSLQDRVIALETSTQVALDHLESVPEKLSLLEDFKDFRDSCSSSERT
DGRYSKYRVRRNSLQHHQDDTKYRTKSFKGDRTFLEGSHTRGLDHSSSWQ
DHSRFLSSPRFSYVNSFTKRTVAPDSASNKEDATMNGTSSQPK
  
EEMGSLQDRVIALETSTQVALDHLESVPEKLSLLEDFKDFRDSCSSSERT
DGRYSKYRVRRNSLQHHQDDTKYRTKSFKGDRTFLEGSHTRGLDHSSSWQ
DHSRFLSSPRFSYVNSFTKRTVAPDSASNKEDATMNGTSSQPK
  
KEKSEAENHIRTLKAESLEEKNMAKIHRGQLEKLKSQCDRLTEELTQNEN
ENKKLKLKYQCLKDQLEEREKHISIEEEHLRRMEEARLQLKDQLLCLETE
QESILGVIGKEIDAACKTFSKDSVEKLKVFSSGPDIHYDP
 

Matching transcripts

 CEP128-001 - ENSP00000451162 [100%]
CEP128-015 - ENSP00000451501 [100%]
CEP128-201 - ENSP00000281129 [100%]
  CEP128-001 - ENSP00000451162 [100%]
CEP128-015 - ENSP00000451501 [100%]
CEP128-201 - ENSP00000281129 [100%]
  CEP128-001 - ENSP00000451162 [100%]
CEP128-201 - ENSP00000281129 [100%]
 

Other gene match

      
ANTIGEN VIEW
CEP128-001
CEP128-015
CEP128-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA000602

  

Antibody HPA001116

  

Antibody HPA018498

  

1.

   Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods
Jakobsen L et al
EMBO J 2011;30(8):1520-35
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.