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NELFCD

ANTIBODY INFORMATION
 
 

Antibody HPA050641

  

Antibody HPA066262

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA050641   HPA066262  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA050641, RRID:AB_2681198   Atlas Antibodies Cat#HPA066262, RRID:AB_2685644  

VALIDATION SUMMARY
 
 

Antibody HPA050641

  

Antibody HPA066262

  
Immunocytochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA050641

  

Antibody HPA066262

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nuclear bodies & vesicles.   Immunofluorescent staining of human cell line MCF7 shows localization to nuclear bodies.  

Antibody dilution

 1:72   1:39  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA050641

  

Antibody HPA066262

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 67.3, 52.4   67.3, 52.4  

Antibody dilution

 1:360   1:130  

PROTEIN ARRAY
 
 

Antibody HPA050641

  

Antibody HPA066262

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4000   1:1550  

ANTIGEN INFORMATION
 
 

Antibody HPA050641

  

Antibody HPA066262

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 80   89  

Antigen sequence

 
ETVENHLKSLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTWRDLFYKL
AEAHPDCLMLNFTVKLISDAGYQGEITSVS
  
KRVSINKDELKSTSKAVETVHNLCCNENKGASELVAELSTLYQCIRFPVV
AMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCH
 

Matching transcripts

 NELFCD-003 - ENSP00000473290 [100%]
NELFCD-201 - ENSP00000342300 [100%]
  NELFCD-003 - ENSP00000473290 [100%]
NELFCD-201 - ENSP00000342300 [99%]
 

Other gene match

    
ANTIGEN VIEW
NELFCD-003
NELFCD-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.