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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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CST3
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    TISSUE

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    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

CST3

Gene description

Cystatin C

Protein class

Cancer-related genes
Disease related genes
Plasma proteins
Predicted secreted proteins

Predicted localization

Secreted

Number of transcripts

3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Mixed
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in several tissues combined with distinct extracellular positivity, most abundant in the salivary gland, epididymis and renal tubules.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Supported based on 2 antibodies.
HPA013143 , CAB000118
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in endometrial cancer (favourable) and urothelial cancer (favourable).
Endometrial cancer p<0.001
Urothelial cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA013143
CAB000118

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A majority of malignant cells displayed moderate to strong cytoplasmic positivity. Malignant lymphoma and renal cancers as well as several cases of lung, skin and liver cancers were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancers showed moderate to strong cytoplasmic positivity. Melanomas, lymphomas, skin and testicular carcinomas were generally negative.

GENE INFORMATION

Gene name

CST3

Synonyms

Description

Cystatin C (HGNC Symbol)

Entrez gene summary

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein. [provided by RefSeq, Nov 2014]

Chromosome

20

Cytoband

p11.21

Chromosome location (bp)

23626706 - 23638473

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000101439 (version 83.38)

Entrez gene

1471

UniProt

P01034 (UniProt - Evidence at protein level)

neXtProt

NX_P01034

Antibodypedia

CST3 antibodies

PROTEIN BROWSER

CST3-001
CST3-002
CST3-003

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

CST3-001
ENSP00000366124
ENST00000376925
P01034 [Direct mapping]
Cystatin-C
Show all
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001540 [beta-amyloid binding]
GO:0002020 [protease binding]
GO:0004866 [endopeptidase inhibitor activity]
GO:0004869 [cysteine-type endopeptidase inhibitor activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0006952 [defense response]
GO:0010466 [negative regulation of peptidase activity]
GO:0010711 [negative regulation of collagen catabolic process]
GO:0010716 [negative regulation of extracellular matrix disassembly]
GO:0010951 [negative regulation of endopeptidase activity]
GO:0034103 [regulation of tissue remodeling]
GO:0042802 [identical protein binding]
GO:0043206 [extracellular fibril organization]
GO:0044267 [cellular protein metabolic process]
GO:0045861 [negative regulation of proteolysis]
GO:0060311 [negative regulation of elastin catabolic process]
GO:0060313 [negative regulation of blood vessel remodeling]
GO:0070062 [extracellular exosome]
Show all
146 aa
15.8 kDa
Yes 0
CST3-002
ENSP00000381448
ENST00000398411
P01034 [Direct mapping]
Cystatin-C
Show all
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001540 [beta-amyloid binding]
GO:0001654 [eye development]
GO:0001666 [response to hypoxia]
GO:0001775 [cell activation]
GO:0002020 [protease binding]
GO:0004866 [endopeptidase inhibitor activity]
GO:0004869 [cysteine-type endopeptidase inhibitor activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005604 [basement membrane]
GO:0005615 [extracellular space]
GO:0005737 [cytoplasm]
GO:0005764 [lysosome]
GO:0005771 [multivesicular body]
GO:0005783 [endoplasmic reticulum]
GO:0006915 [apoptotic process]
GO:0006952 [defense response]
GO:0006979 [response to oxidative stress]
GO:0007420 [brain development]
GO:0007431 [salivary gland development]
GO:0007566 [embryo implantation]
GO:0008284 [positive regulation of cell proliferation]
GO:0008584 [male gonad development]
GO:0009636 [response to toxic substance]
GO:0009743 [response to carbohydrate]
GO:0010035 [response to inorganic substance]
GO:0010466 [negative regulation of peptidase activity]
GO:0010711 [negative regulation of collagen catabolic process]
GO:0010716 [negative regulation of extracellular matrix disassembly]
GO:0010951 [negative regulation of endopeptidase activity]
GO:0014070 [response to organic cyclic compound]
GO:0030414 [peptidase inhibitor activity]
GO:0030424 [axon]
GO:0031667 [response to nutrient levels]
GO:0031965 [nuclear membrane]
GO:0031982 [vesicle]
GO:0032355 [response to estradiol]
GO:0034103 [regulation of tissue remodeling]
GO:0034599 [cellular response to oxidative stress]
GO:0042493 [response to drug]
GO:0042747 [circadian sleep/wake cycle, REM sleep]
GO:0042802 [identical protein binding]
GO:0042995 [cell projection]
GO:0043025 [neuronal cell body]
GO:0043067 [regulation of programmed cell death]
GO:0043206 [extracellular fibril organization]
GO:0043292 [contractile fiber]
GO:0044267 [cellular protein metabolic process]
GO:0045740 [positive regulation of DNA replication]
GO:0045861 [negative regulation of proteolysis]
GO:0048471 [perinuclear region of cytoplasm]
GO:0048678 [response to axon injury]
GO:0060009 [Sertoli cell development]
GO:0060311 [negative regulation of elastin catabolic process]
GO:0060313 [negative regulation of blood vessel remodeling]
GO:0060548 [negative regulation of cell death]
GO:0070062 [extracellular exosome]
GO:0070301 [cellular response to hydrogen peroxide]
Show all
146 aa
15.8 kDa
Yes 0
CST3-003
ENSP00000381446
ENST00000398409
P01034 [Direct mapping]
Cystatin-C
Show all
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001540 [beta-amyloid binding]
GO:0002020 [protease binding]
GO:0004866 [endopeptidase inhibitor activity]
GO:0004869 [cysteine-type endopeptidase inhibitor activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0006952 [defense response]
GO:0010466 [negative regulation of peptidase activity]
GO:0010711 [negative regulation of collagen catabolic process]
GO:0010716 [negative regulation of extracellular matrix disassembly]
GO:0010951 [negative regulation of endopeptidase activity]
GO:0034103 [regulation of tissue remodeling]
GO:0042802 [identical protein binding]
GO:0043206 [extracellular fibril organization]
GO:0044267 [cellular protein metabolic process]
GO:0045861 [negative regulation of proteolysis]
GO:0060311 [negative regulation of elastin catabolic process]
GO:0060313 [negative regulation of blood vessel remodeling]
GO:0070062 [extracellular exosome]
Show all
146 aa
15.8 kDa
Yes 0

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