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NOL4

ANTIBODY INFORMATION
 
 

Antibody HPA046740

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046740  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 9  

References

  

Proper citation

  

VALIDATION SUMMARY
 
 

Antibody HPA046740

  
Immunocytochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046740

  

Standard validation

 Uncertain  

 

Figure description

 Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus.  

Antibody dilution

 1:29  

Literature conformity

 The subcellular location is not consistent with literature.  

WESTERN BLOT
 
 

Antibody HPA046740

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 71.4, 61.3, 59.3  

Antibody dilution

 1:150  

PROTEIN ARRAY
 
 

Antibody HPA046740

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1150  

ANTIGEN INFORMATION
 
 

Antibody HPA046740

  

Antigen

 Recombinant protein fragment  

Length (aa)

 70  

Antigen sequence

 
KYERIVQLLNGSESSSTDNAKFKFWVKSKGFQLGQPDEVRGGGGGAKQVL
YVPVKTTDGVGVDEKLSLRR
 

Matching transcripts

 NOL4-001 - ENSP00000261592 [100%]
NOL4-002 - ENSP00000465450 [100%]
NOL4-003 - ENSP00000467629 [100%]
 

Other gene match

  
ANTIGEN VIEW
NOL4-001
NOL4-002
NOL4-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.