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Immunofluorescent staining of human cell line HeLa shows localization to nucleus.
Antibody dilution
1:81
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA067703
Antibody CAB005363
Standard validation
Approved
Figure description
Immunohistochemical staining of human pancreas shows distinct nuclear positivity in exocrine glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
Antibody dilution
1:35
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA067703
Antibody CAB005363
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
62.5, 8.8, 6.3
62.5, 57, 8.8, 8.2, 6.3, 5
Antibody dilution
1:270
1:500
PROTEIN ARRAY
Antibody HPA067703
Antibody CAB005363
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
